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. 2009 Nov 30;9:416. doi: 10.1186/1471-2407-9-416

Figure 3.

Figure 3

Western blotting analysis to detect indoleamine 2,3-dioxygenase (IDO) expression in the nasopharyngeal carcinoma cell line CNE2 induced by activated peripheral blood lymphocytes (PBLs). (A) CNE2 cells were treated with or without 100 U/ml IL-2 or 50 U/ml IFNγ for 24 h. (B) PBLs were stimulated with 100 U/ml IL-2 for 96 h, washed to remove early generated cytokines and cultured in fresh complete RPMI 1640 medium supplemented with 100 U/ml IL-2 for 24 h. The PBL supernatant was then used as the CM to incubate CNE2 cells for 24 h. (C) PBLs were stimulated with 100 U/ml IL-2 for 96 h and washed to remove early generated cytokines. After further incubation with IL-2-supplemented CNE2-CM from CNE2 cells treated or untreated with IFNγ for 24 h, the PBL supernatants were collected and used to incubate CNE2 cells for 24 h. Expression of IDO in CNE2 cells was detected by western blotting analysis with anti-IDO antibodies. The data are representative of three individual experiments. CM, conditioned medium; CNE2-CM, conditioned medium derived from CNE2 cells.