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. 2010 Jan;24(1):128–135. doi: 10.1096/fj.09-137091

Figure 1.

Figure 1.

Expression of SMDR2 in stably transfected CHO cells. A) RT-PCR amplification of SMDR2 from CHO cells stably transfected with SMDR2 or the G418-sensitive parental control line. SMDR2 template was used as a positive control. B) Western blot analysis of Pgp or β-actin from membrane vesicles isolated from SMDR2-tranfected CHO cells or the parental control line. Anti-Pgp monoclonal antibody (C-219) recognizes a 140- to 150-kDa protein from the SMDR2-transfected CHO cell line but not from the parental control line.