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. 2010 Jan;24(1):275–285. doi: 10.1096/fj.09-137158

Figure 4.

Figure 4.

AICAR causes bone loss and elevated bone turnover. Male C57Bl/6 mice (10 wk old) were treated with AICAR (500 mg/kg/d) or saline for 4 wk. A, B) pQCT analysis of femoral trabecular density (A) and femoral cortical thickness (B). C) Low-power views of undecalcified sections of tibia from saline and AICAR-treated mice histochemically stained by von Kossa method (black indicates mineralized bone) and counterstained with ponceau-orange G. D) Tibial trabecular volume, BV/TV. E) Tibial trabecular numbers/mm (Tb.N). F) Tibial trabecular thickness (Tb.Th). G) Tibial OC numbers on trabecular bone (Oc.N). H) Surface of tibial bone occupied by OBs, Ob.S/BS. I) Tibial osteoid volume (OV/BV). **P < 0.01, ***P < 0.001 vs. saline control (black columns); n = 6 mice/treatment group.