Figure 7.

CD induces hypermethylation of a specific CpG site within Calb1 gene. DNA pyrosequencing after bisulfite treatment indicates that CD induces specific hypermethylation of the CpG site 3 within the CpG island of Calb1 promoter (CD 16.9±0.2 vs. CT 14.2±0.2%). CpG sites 7–9 are located within the RE1 binding site (RE1 sequence underscored in inset). Gray bars, CD; black bars, CT. Error bars = se. *P < 0.00555. Each CpG site is marked with bold letters. Arrow indicates transcription start site for Calb1.