Table 1. Primer sequences used for PCR amplification.
Gene | Primer (5′-3′) |
---|---|
rd1 |
F: TGACAATTACTCCTTTTCCCTCAGTCTG |
R: GTAAACAGCAAGAGGCTTTATTGGGAAC | |
Reverse wt primer: TACCCACCCTTCCTAATTTTTCTCACGC | |
D1 |
F: CTGATTAGCGTAGCATGGACTTTGTC |
R: TGGATGTGGAATGTGTGCGAG | |
Reverse wt primer: TGGTGGCTGGAAAACATCAGA | |
D2 |
F: TGATGACTGCGAATGTTGGTGTGC |
R: AGGATTGGGAAGACAATAGCAG | |
Reverse wt primer: CGGAGCCAAGCTAACACTGCAGAG | |
D4 |
F: GCCCGGTTCTTTTTGTCAAG |
Forward wt primer: CATGGACGTCATGCTGTGCA | |
R: CGGACGAGTAGACCACATAG | |
D5 | F: ACTCTCTTAATCGTCTGGACCTTG |
R: GTTCAGATCCGCCGTATCTCCTCC |