Materials	6.5% (vol/vol) resolving	10% (vol/vol) resolving	12.5% (vol/vol) resolving	15% (vol/vol) resolving	5% (vol/vol) stacking
40% (wt/vol) acrylamide	3.1 ml	4.8 ml	6.0 ml	7.1 ml	1.2 ml
2% (wt/vol) bis-acryl	1.7 ml	2.7 ml	3.4 ml	4.0 ml	0.7 ml
1 M Tris, pH 8.7	7.5 ml	7.5 ml	7.5 ml	7.5 ml	—
1MTris, pH6.9	—	—	—	—	1.5ml
10% (wt/vol) SDS	200 μl	200μl	200μl	200μl	125 μl
ddH2O	7.4 ml	4.7 ml	2.8 ml	1.0 ml	8.9 ml
10% (wt/vol) APS	100 μl	100μl	100μl	100μl	63μl
TEMED	10 μl	10μl	10μl	10μl	13μl