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. 2009 Nov 26;28(24):3832–3844. doi: 10.1038/emboj.2009.351

Figure 4.

Figure 4

The RevCen fragment. (A) Representation of siRNA from wild type (WT) and rpb7G150D cells at otr2L-dg according to their position and orientation. (B) Histogram of siRNA size distributions from WT and rpb7G150D cells, siRNA length in nucleotides on x-axis, and percentage of number of siRNA on y-axis. (C) Sequence alignment of the RevCen promoter region characterized in Djupedal et al. (2005) in the dg repeats from all centromeres. Coordinates are: otr1L-dg from 3752406, otr1R-dg from 3778228, otr2L-dg from 1604242, otr3L-dg from 1073094, and otr3R-dg from 1137620. The region is not present in otr2R-dg. The arrow indicates the direction of transcription. (D) Sequence alignment showing the 340-nt translocation present in all dh and dg elements from the three centromeres, one dh and dg repeat per chromosome arm is shown as they are identical. The siRNAs VII and VIII are shown in boxes with orientation. Coordinates are: otr1R-dh from 3781697, otr2L-dh from 1611493, otr2R-dh from 1636391, otr3L-dh from 1088967, otr3R-dh from 1109980, otr1L-dg from 3763484, otr1R-dg from 3784652, otr2L-dg from 1604792, otr3L-dg from 1082192, and otr3R-dg from 1116691. The region is not found in otr1L-dh and otr2R-dh.