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. 2010 Jan 5;5(1):e8426. doi: 10.1371/journal.pone.0008426

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Fittipaldi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(Available at the Sanger Institute, http://www.sanger.ac.uk/Projects/S_suis/). The srtF pilus cluster comprises 4 genes: sipF encoding a signal peptidase; sfp2, which is now considered to be a pseudogene formed by the previously reported coding sequences SSU_0425 and SSU_0426 (indicated by the thick dark grey arrows); sfp1, encoding the major pilin subunit; and srtF, encoding a dedicated (pilin polymerase) sortase. Small arrows indicate the annealing positions of the primers used to generate the 6xHis recombinant proteins rSfp1, rSfp2A and rSfp2B. The dashed lines surrounded by arrows indicate the regions deleted in the ΔsipF, Δsfp2, Δsfp1 and ΔsrtF mutants.