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. 2010 Jan 5;5(1):e8426. doi: 10.1371/journal.pone.0008426

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Fittipaldi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mutanolysin-extracted surface proteins were resolved on Tris-HCl Ready gradient 4–15% SDS-PAGE gels (BioRad) and transferred onto nitrocellulose membranes. Sfp1 was detected using specific rabbit antisera and horseradish peroxidase-coupled, goat anti-rabbit secondary antibodies (Jackson ImmunoResearch, West Grove, PA). Five µg of protein were loaded per well. M: Molecular weight markers. Lane 1: WT strain P1/7. Lane 2: ΔsipF mutant. Lane 3: Δsfp2 mutant. Lane 4: Δsfp1 mutant. Lane 5: ΔsrtF mutant. See the results section for more details.