|
E. coli
|
| TOP10 |
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG |
Invitrogen |
| BL21λDE3 |
F−
ompT gal (dcm) (lon) hsdSB(rB
− mB
−) endA1 hsdR17(rK
−mK
+) |
Invitrogen |
|
S. suis serotype 2 |
| P1/7 |
Virulent strain isolated from a pig with meningitis |
[58]
|
| 31533 |
Virulent strain isolated from a pig with meningitis |
[10]
|
| 166′ |
Virulent strain isolated from a pig with meningitis |
[59]
|
| D282 |
Virulent strain isolated from a diseased pig |
[60]
|
| S735 |
Serotype 2 reference strain, isolated from a pig with pneumonia |
[61]
|
| 24 |
Virulent strain isolated from a human case of meningitis |
[62]
|
| LEF95 |
Virulent strain isolated from a human case of meningitis |
[63]
|
| HUD Limoges |
Virulent strain isolated from a human case of septic-shock |
[64]
|
| 89–1591 |
Virulent field strain isolated from a pig with septicemia |
[65]
|
| ΔsipF
|
Derived from P1/7. In frame deletion of sipF
|
This work |
| Δsfp2
|
Derived from P1/7. In frame deletion of sfp2
|
This work |
| Δsfp1
|
Derived from P1/7. In frame deletion of sfp1
|
This work |
| ΔsrtF
|
Derived from P1/7. In frame deletion of srtF
|
This work |
| ΔsrtA
|
Derived from P1/7. Deletion of srtA
|
This work |
| ΔsrtAcompsrtA
|
ΔsrtA complemented with pSAcomp1 |
This work |
| ΔsrtAcompmock |
ΔsrtA transformed with empty pSET-3 |
This work |
| Plasmids |
| pCR2.1 |
Apr, Kmr, oriR(f1) MCS oriR (ColE1) |
Invitrogen |
| pDIA17 |
Cmr, oriR pACYC184, Tet promoter ΔlacI
|
[66]
|
| pIVEX2.4d |
Apr, oriR pUC, T7 promoter, His-Tag coding sequence |
Roche Applied Science |
| pSET4s |
Thermosensitive vector for allelic replacement is S. suis. Replication functions of pG+host3, MCS oriR pUC19 lacZ SpR
|
[49]
|
| p4ΔsipF
|
pSET4s carrying the construct for sipF allelic replacement |
This work |
| p4Δsfp2
|
pSET4s carrying the construct for sfp2 allelic replacement |
This work |
| p4Δsfp1
|
pSET4s carrying the construct for sfp1 allelic replacement |
This work |
| p4ΔsrtF
|
pSET4s carrying the construct for srtF allelic replacement |
This work |
| pSAD1 |
pSET4s carrying cat flanked by 5′ and 3′ ends of srtA
|
[43]
|
| pSAD11 |
pSAD1 devoid of cat
|
This work |
| pSET-3 |
S. suis-E. coli shuttle vector |
[50]
|
| pSAcomp1 |
pSET-3 carrying the full length srtA gene under the control of cat promoter |
[43]
|
