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. 2010 Jan 5;5(1):e8468. doi: 10.1371/journal.pone.0008468

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Field et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cells transformed with the p2T7 RNAi plasmid harboring an insert corresponding to the indicated gene, were analyzed for the effects of RNAi on replication. Cell numbers were determined daily, and cultures diluted as necessary (see methods). Panel A; HSP class, Panel B; Lectin/glycosidase class, and Panel C; others. Blue lines are data from uninduced cultures and red lines from induced. A single data set is shown, which is representative of three or more independent analyses on separate clones. Data for Tb927.4.4740 (Lag1) are not shown. Tb11.02.4100 (Sec61) and Tb11.02.5450 (BiP) are included as positive controls. RNAi clones scored as having normal growth are on a gray background. Note that in some instances the uninduced cultures appear to grow at slower rate when compared to untransfected (not shown) or cells harboring an RNAi construct with no apparent replication impact.