Table 1. ER chaperones and ER quality control gene products in Trypanosoma brucei.
| Accession (geneDB) | Annotation | Functional assignment | T. brucei status1 |
| HSP and DNAj | |||
| 11.02.5450/55002 | BiP | Major ER chaperone | Orthologue |
| 09.160.30903 | Hsp70 | Chaperone | Paralogue (SS, degenerate HDEL) |
| 09.211.1390 | Hsp70 | Chaperone | Paralogue |
| 11.01.31104 | Hsp70 | Chaperone | Paralogue |
| 927.3.3580 | Endoplasmin/GRP94/LPG3 | Secretory pathway chaperone | Orthologue (SS, degenerate HDEL) |
| 927.3.1430 | DNAj | Chaperone | SS, DNAj domain |
| 11.01.8480 | DNAj | Chaperone | SS, DNAj domain |
| 09.211.3680 | DNAj | Chaperone | SS, DNAj domain |
| 10.70.5440 | DNAj | Chaperone | SS, DNAj domain |
| 09.211.1550 | Sec63 | Part of DNAj family | Divergent orthologue [54] |
| Glucosylation and lectins | |||
| Calnexin | Quality control | Not found | |
| 927.4.5010/927.8.74102 | Calreticulin | Quality control | Orthologue |
| ER glucosidase I | Quality control | Not found | |
| 10.05.0080 | ER glucosidase II | Quality control | Orthologue |
| 927.8.2910/2920/2930/29405 | EDEM | Quality control | Orthologue |
| 927.3.4630 | Glycoprotein glucosyltransferase I | Quality control | Orthologue |
| 11.01.2470 | Yos9 | Quality control | Orthologue |
| 11.02.1680 | ERGIC53 | Selective quality control | Orthologue |
| 10.20.0130 | Vip36 | ERGIC53 paralogue | Orthologue |
| Protein disulfide isomerase system | |||
| 927.7.1300 | ERp72-like | Cofactor with calnexin, PDI activity | Orthologue |
| 10.6k15.2290 | ERp57-like | Cofactor with calnexin, PDI activity | Orthologue |
| 927.8.4890 | Ero1 | Oxidizes PDI | Orthologue |
| 927.7.5790 | ERp44-like | Complexes with Ero1, oxidoreductase | Orthologue |
| GPI/lipid transport class | |||
| 927.4.4740 | Lag1/Dgt1 | ER to Golgi transport of GPI proteins | Orthologue |
| CERT | ER to Golgi transport of ceramides | Not found | |
| Translocon (control) | |||
| 11.02.4100 | Sec61 | Part of ER translocation channel | Orthologue |
Gene products were identified by searching the trypanosome genome database as described in methods.
Indicates presence of orthologue that fulfils criteria of reverse blast to higher eukaryote sequence, correct domain and sequence feature retention, or not found, i.e. fails criteria. Note that not found does not necessarily mean that a gene product with similar function to the higher eukaryote query sequence is not present. Paralogues indicates multiple distinct genes found, and based on sequence alone orthology cannot be unambiguously established.
BiP: Tb11.02.5450 and Tb11.02.5500 ORF sequences are identical. Calreticulin: Tb927.4.5010 (chromosome 4) and Tb927.8.7410 (chromosome 8) ORF sequences are identical.
Hsp70 (Tb11.01.3110) - no signal peptide; C-terminal ER retention motif is very degenerate (SSSL).
Hsp70 (Tb09.160.3090) - C-terminal ER retention motif is very degenerate (LKDLK LGE).
EDEM: cluster of four genes: Tb927.8.2910, 2920 and 2930 are near identical, while Tb927.8.2940 is C-terminally truncated, suggesting two EDEM paralogues; designated class I and II respectively. Two RNAi constructs were designed, EDEM A and B, which will target both isotypes due to high DNA sequence conservation over much of the ORF.