Table 2. Summary of phenotypic effects obtained by RNAi of Trypanosoma brucei ER chaperone candidates.

Function	Number1	Proliferation2	Morphology2	ER2	Increase in VSG abundance2
Chaperone/HSP	8	63	63	63	38
Calreticulin/redox	11	80	80	80	72
Post-ER lectins	2	50	50	50	50
GPI/sphingolipid	1	0	0	0	0
Total	22	68	68	68	55

Each ORF was subjected to suppression using RNA interference, and the effect on proliferation, cell morphology, ER morphology (ER) and the presence of increased VSG abundance monitored as described in methods and the text. ORFs are grouped in functional classes, using the same categories as in Table 1.

¹Total number of distinct ORFs analysed; number collapsed to one for multiple copies targeted by same construct.

²Data for phenotype are expressed as percent of total number of ORFs tested.