Table 1.

Oligonucleotides

	Sequence (5′ → 3′)
Probe 1	CCACTGTTGCAAAGTTATT (T)10--|
Probe 2	CGCTTCTGTATCTATATCATCA (T)10--|
Complement 1	AATAACTTTGCAACAGTGG-
	AATAACTTTGCAACAGTGG
Complement 2	TGATGAATATAGATACAGAAGCG-
	TGATGAATATAGATACAGAAGCG

Probe oligonucleotides used in this work were synthesized directly onto hydroxyl-terminated substrates (3′ → 5′ direction) using NPPOC-protected phosphoramidite bases and in situ maskless array synthetic methods. Each probe oligonucleotide is separated from the surface (–|) by 10 thymidine (dT) residues. Complement oligonucleotides were synthesized using standard phosphoramidite chemistries. Complement oligonucleotides with 3′-fluorophore modifications were used in fluorescence-based experiments and complement oligonucleotides without 3′-modifications were used in SPR-based experiments. Complement 1 is modified with a 3′-fluorescein moiety and Complement 2 with a 3′-Cy5 moiety.