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. 2010 Jan 5;5(1):e8567. doi: 10.1371/journal.pone.0008567

Figure 3. Effect of 2-Bromopalmitate on GD3/CLIPR-59 association.

Figure 3

Quantitative evaluation of GD3/CLIPR-59 association by FRET technique, as revealed by flow cytometry analysis. This association, negligible in untreated cells, started 15 min after the administration of anti-CD95/Fas (250 ng/ml for different incubation times at 37°C) reached its peak 30 min later and dropped down 90 min after treatment (left column). In cell pre-treated with 100 µM 2-Bromopalmitate for 3 h at 37°C to inhibit palmitoylation, GD3/CLIP-59 association was inhibited at any time (right column). Numbers represent the percentage of FL3 positive cells indicating the GD3/CLIPR-59 association. Results obtained in one experiment representative of three are shown. (B) Time course analysis of the association GD3/CLIPR-59 obtained pooling together results of three independent experiments. Data are reported as mean values±SD.