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. 2009 Oct 6;284(49):33740–33749. doi: 10.1074/jbc.M109.047779

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Functional reconstitution of TAP in proteoliposomes. Proteoliposomes (46 μg of E. coli lipids) containing wt (filled bars) or inactive TAP (open bars; 0.75 μg of each) were incubated in 50 μl with 1 μm RRYC^(F)KSTEL in the presence of MgATP or MgADP (3 mm of each). The assay was performed in the presence of unlabeled competitor peptide (200 μm RRYQKSTEL), the viral TAP inhibitor ICP47 (30 μm), or ortho-vanadate (3 mm) as indicated. Data are derived from triplicate measurements. Error bars show the standard deviation.