TABLE 1.

Efficiency of detergents in solubilization und purification

	TAP1/TAP2 solubilization efficiencya	TAP1/TAP2 recovery after purificationa	TAP2/TAP1 ratio after purificationb
	%	%
FC-14	75/95	30/1	0.1
DDM	88/94	68/69	1.0
TX-100	69/74	69/75	1.0
Digitonin	77/96	36/31	1.0

^a To determine the efficiency in solubilization and recovery of TAP, the amount of TAP in crude membranes was compared with TAP after solubilization and purification by densitometry of immunoblots using monoclonal α-TAP1 (148.3) and α-TAP2 (435.3) antibodies.

^b The molar ratio of TAP1 and TAP2 in crude membranes was taken as 1.