TABLE 3.
Parameters derived from fluorescence auto-correlation spectroscopy
| Excitation wavelength | 488 nm | 633 nm |
| Dye | Alexa-488 | Alexa-633 |
| τdye water/buffer (μs) | 35/86 | 53/109 |
| Ddye water/buffer (μm2/s) | 430a/175 | 370b/180 |
| Confocal volume (fl) | 0.5 | 1.1 |
| Viscosity of buffer 20 °C (*103 N*s/m2) | 2.5 | 2.2 |
| Structure parameter, S | 5.8 | 9 |
| τpeptide water/buffer (μs) | 50/120 | 94/190 |
| Dpeptide water/buffer (μm2/s) | 250/104 | 209/103 |
| Confocal volume (fl) | 0.5 | 1.1 |
| Particle number | 6.3 ± 0.5 | 7.4 ± 3.6 |
| Peptide concentration (nm) | 21.8 | 11.2 |
| RH peptide (Å) | 8.4 | 7.0 |
| τpeptide TAP (μs) | 1500 | 2000 |
| Dpeptide TAP (μm2/s) | 10 | 10 |
| RH (Å) | 87.6 | 73.7 |
| RS (Å) | 35.1 | 35.1 |
| RH/RS | 2.5 | 2.1 |