FIGURE 1.
The role of secretion and Gi signaling in Akt phosphorylation by CVX in human platelets. A, platelets preincubated with 100 nm AR-C69931MX, 100 μm MRS2179, 25 μm LY294002, 10 μm dimethyl-BAPTA, 10 μm PP2, or 10 μm GF109203X were stimulated at 37 °C for 3 min with 100 ng/ml CVX. Equal amounts of proteins were separated by SDS-PAGE, Western blotted, and probed for anti-phospho-Akt (Ser473 and Thr308) or anti-β-actin (lane loading control) antibody. B, densitometric measurement of phospho-Akt (Ser473) and phospho-Akt (Thr308), expressed as percentage of agonist control. The blot shown is a representative of three independent experiments. Data are mean ± S.E. (n = 3); *, p < 0.01 compared with agonist.