FIGURE 4.

Selective stimulation of either G_i- or G_z-coupled receptor restores CVX-induced Akt phosphorylation upon blockade of secretion. A, platelets preincubated in the absence and presence of 10 μm GF109203X were stimulated at 37 °C for 3 min with 100 ng/ml CVX. The addition of 100 nm 2-MeSADP or 10 μm epinephrine was made as indicated. B, platelets isolated from P2Y₁₂^−/− mice were stimulated at 37 °C for 3 min with 100 ng/ml CVX in the absence and presence of 10 μm epinephrine. Platelet proteins were separated by SDS-PAGE, Western blotted, and probed for anti-phospho-Akt (Ser⁴⁷³) or anti-β-actin (lane loading control) antibody.