FIGURE 6.

The effect of PI3K inhibitors on CVX-induced platelet aggregation, secretion, and Ca²⁺ mobilization. Washed platelets preincubated with 25 μm LY294002, 100 nm PIK75, 500 nm TGX-221, 2 μm AS-252424, or 1 μm IC87114 were stimulated at 37 °C with 100 ng/ml CVX in the presence of 100 nm AR-C69931MX and 10 μm MRS-2179, and platelet aggregation (A), ATP secretion (picomoles/10⁸ platelets) (B), 5-HT secretion (C), and Ca²⁺ mobilization (D) were measured as noted. Tracings (A) are representative of results obtained from three different donors. The results (B–D) show composite data from three experiments, and values are mean ± S.E. (n = 3); *, p < 0.05 compared with CVX.