FIGURE 1.
HxcQ forms SDS and heat-resistant HMM complexes. Immunodetection of HxcQ secretin with anti-HxcQ multimers and anti-HxcQ monomer antibodies. The PAO1 hxcQ mutant complemented with pJNhxcQV5 was grown under standard conditions to induce HxcQV5 production. Whole cell extracts were collected, resuspended in SDS-PAGE sample buffer containing 2% SDS and incubated at the indicated temperature for 10 min. The proteins were separated using 3.5% acrylamide stacking and 9% running gel. The positions of HxcQ multimers and monomers are indicated on the left.