FIGURE 6.
Differential solubilization of membrane-associated HxcQV5 and HxcQnlV5. Membrane fractions containing HxcQV5 or HxcQnlV5 were treated with 2% (v/v) Triton X-100, 100 mm sodium carbonate, pH 11, or 4 m urea at pH 6.5 for differential solubilization. Soluble and insoluble fractions were analyzed under semi-native conditions for HxcQV5 and HxcQnlV5 multimer detection and under denaturing conditions on a 12% (w/v) SDS-polyacrylamide gel for XcpR and XcpY. HxcQV5 and HxcQnlV5 were probed with anti-V5 antibody. Molecular masses (in kDa) are indicated on the left.