TABLE 1.
Strains and plasmids used in this study
Ap, ampicillin; Gm, gentamicin; Km, kanamycin; Sm, streptomycin.
| Strains, vectors, and plasmids | Relevant characteristics | Source or Ref. |
|---|---|---|
| Strains | ||
| E. coli | ||
| TG1 | SupE Δ(lac-proAB) thi hsd RΔ5 (F':traD36 rpo A+B+ lacIq lacZΔM15) | Lab collection |
| DH5α | supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Lab collection |
| CC118λpir | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 RfR(λpir) | Ref. 46 |
| P. aeruginosa | ||
| PAO1 | Prototrophe, chl-2 | B. Holloway |
| PAO1ΔxcpQ | Non polar deletion of the xcpQ gene in PAO1 | Ref. 47 |
| PAO1ΔhxcQ | Non polar deletion of the hxcQ gene in PAO1 | This study |
| PAO1ΔhxcQΔxcpQ | Non polar deletion of the hxcQ gene in PAO1ΔxcpQ | This study |
| PAO1ΔhxcQΔxcpQΔpAΔqA | Non polar deletion of the xphA, and xqhA genes in PAO1ΔhxcQxcpQ | This study |
| Vectors and Plasmids | ||
| pKNG101 | SmR, mobRK2, sacBR+ (suicide vector) | Ref. 48 |
| pRK2013 | KmR, ColE1, Tra+ Mob+ (RK2) | Ref. 30 |
| pCR2.1 | ApRKmR, ColE1, f1 ori | Invitrogen |
| pJN105 | GmR, araC-pBAD, (broad-host-range vector) | Ref. 49 |
| pMMB67HE | ApR, IncQ tac promoter; lacIq, (broad-host-range vector) | Ref. 50 |
| pMMB67HEhxcQV5 | hxcQV5 cloned into pMMB67HE (XbaI/SmaI) | This study |
| pMB4 | xcpQ cloned in pMMB67HE (PstI-SmaI) | Ref. 47 |
| pET-DEST42 | ApR CmR, Gateway destination vector | Invitrogen |
| pET-DEST42xcpQV5 | xcpQV5 cloned in the Gateway vector pET-DEST42 | This study |
| pET-DEST42hxcQV5 | hxcQV5 cloned in the Gateway vector pET-DEST42 | This study |
| pKNGΔhxcQ | Mutator plasmid for hxcQ deletion | This study |
| pKNGΔpAqA | Mutator plasmid for xphA-xqhA deletion | Ref. 31 |
| pJNhxcQV5 | hxcQV5 cloned into pJN105, pBAD | This study |
| pJNhxcQnlV5 | hxcQnlV5 cloned into pJN105, pBAD | This study |
| pJNhxcQnl | hxcQnl cloned into pJN105, pBAD | This study |