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Measurement of Δψ in cells producing HxcQnlV5 or HxcQV5
Accumulation of [3H]-TPP+ was measured by the ratio of radioactivity inside and outside the cells (second column) and used to calculate the Δψ (third column), as described under “Experimental Procedures.”
| Strains | [3H]-TPP+ in/out | Δψ |
|---|---|---|
| mV | ||
| Wild type/pJN105 | 1,000 | 180 ± 4 |
| ΔhxcQ/pJN105 | 1,113.4 | 183 ± 2 |
| ΔhxcQ/pJN105hxcQV5 | 857.7 | 176 ± 8 |
| ΔhxcQ/pJN105hxcQnlV5 | 256 | 145 ± 11 |
