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Strand transfer assay of WT and GAG-mut template under different cation conditions. The template descriptions are the same as in Fig. 2A. Different templates and cations are indicated on the top of the gel. The acceptor RNA used in this assay is WT acceptor RNA. Reactions were terminated at 5, 10, 20, 40 min. Major pause sites are indicated on the left side of the gel. DE, donor extension product; TP, transfer product.
