FIGURE 4.

Cryo-TEM analyses of membrane fractions from cells expressing alMGS and isolated by sucrose gradient separations. A, vesicle fraction from the 27% sucrose step. B,“normal” inner membrane fraction from the 32 to 37% sucrose steps. C, normal outer membrane fraction from the 43 to 49% sucrose step. The black arrowheads indicate inner membrane vesicles, and the black arrows indicate outer membrane fragments in the isolated membrane fractions. The white arrowheads exemplify the appearance of ice crystals deposited on the sample surface after vitrification. Scale bars, 200 nm.