FIGURE 1.
Knockdown of LRP1 in primary neurons. A, titration of LRP1 shRNA lentiviruses targeting two different regions of LRP1. The neurons were infected with 1 × 105, 2.5 × 105, or 1 × 106 transforming units of the indicated lentiviruses, and LRP1 and β-actin levels were analyzed by Western blot. A lentivirus prepared from pLKO.1 empty vector was used as a control. Both LRP1 shRNAs efficiently decreased LRP1 levels. LRP1 shRNA 2 was selected for the remainder of this study. B, triple staining for LRP1 (red), MAP2 (green), and DAPI (blue) in control and LRP1 shRNA-infected neurons. A robust LRP1 staining was detected in the soma and a spotted pattern in MAP2-positive processes and were significantly decreased upon infection with LRP1 shRNA. Scale bar, 50 μm.