FIGURE 3.

Overall apparent growth phenotype of Arabidopsis seedlings expressing AtFAAH mutants in response to NAE and ABA. Seedlings were grown for 10 days in the presence of DMSO only, 30 μm NAE12:0, or 0.25 μm ABA (A). Growth phenotype in liquid media. Seedlings were grown in flasks, and then transferred onto Petri dishes to photograph. Representative images of triplicate experiments are shown here. All site-directed mutant lines (SW, RW, SK, and RK) display no growth enhancement and no NAE tolerance compared with native AtFAAH overexpressor (OE), whereas they all show ABA hypersensitivity compared with their background lines (WT and KO). B, growth phenotype in solid media. Representative images of 24 individual seedlings for each line are shown. Note that relative growths of the different lines are highly analogous to those in liquid medium (A). KO, knock-out; SW, S281A/S282A mutant expressed in wild-type background; RW, R307A mutant expressed in wild-type background; SK, S281A/S282A mutant expressed in Atfaah knock-out background; RK, R307A mutant expressed in Atfaah knock-out background.