FIGURE 5.

Enhanced growth of nonhost pathogen P. syringae pv. tabaci in Arabidopsis expressing AtFAAH mutants. A, leaves were syringe-infiltrated with bacteria of a concentration of 5 × 10⁷ colony-forming units/ml for symptom development. The disease symptoms were photographed at 5 days post-inoculation. Inoculated leaves are indicated by arrows. B, plants were vacuum-infiltrated with bacteria of a concentration of 10⁷ colony-forming units/ml for growth assays. Bacterial growth was quantified at 3 (white bars) and 0 days post-inoculation (black bars) for comparison. The error bars represent S.D. from triplicate measurements. Asterisks indicate a significant difference (p < 0.0001) compared with wild-type (WT; 3 days post-inoculation), which was determined by Student's t test. OE, overexpressor; SW, S281A/S282A mutant expressed in wild-type background; RW, R307A mutant expressed in wild-type background; SK, S281A/S282A mutant expressed in Atfaah knock-out background; RK, R307A mutant expressed in Atfaah knock-out background.