FIGURE 5.

Interaction of CAX half proteins at the tonoplast in plant cells. Onion epidermal cells transiently co-expressing CAX constructs fused to the N-terminal half of YFP (^NYFP) and the C-terminal half of YFP (^CYFP) (left panels) with a CFP-tagged vacuolar marker (middle panels), and the merged image (right panels). Top row panels, co-expression of CAX1-^CYFP and CAX3-^NYFP; middle row panels, co-expression of sCAX1 N-terminal half protein (N-sCAX1)-^NYFP and CAX1 C-terminal half protein (C-CAX1)-^CYFP; bottom row panels, co-expression of N-sCAX1-^CYFP and CAX3 C-terminal half protein (C-CAX3)-^NYFP. Reconstituted YFP fluorescence signal is shown as false color green, CFP fluorescence signal is shown as false color red, and overlapping YFP/CFP fluorescence signal in the merged image is yellow. Bar, 100 μm.