Phylogenetic tree of representative ADP-Glc PPase proteins from photosynthetic eukaryotes. This tree was constructed using maximum likelihood methods where the cyanobacteria Nostoc sp. was used as the outgroup (Nsp., gray). The L subunits were separated into five main groups as follows: group IV (purple), group III (green), group II (yellow), group I (pink), and unicellular algae (blue). Orange is used to show S subunits from monocots, dicots, and unicellular algae. Accession numbers and abbreviations are in supplemental Table 2. Confidence values for each branch calculated by the TREE-PUZZLE program are included in the tree.