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Gel densitometry of OtaS and OtaS/OtaL proteins. The SDS-PAGE shown was used to determine the stoichiometry of the OtaS:OtaL subunits. M corresponds to the molecular weight protein marker; S refers to the OtaS homomeric protein; and S/L is the OtaS/OtaL heteromeric protein. The purified protein loaded in each lane was 1 μg. The electrophoresis and the gel staining proceeded as described under “Experimental Procedures.”
