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. 2009 Sep 8;284(49):34092–34102. doi: 10.1074/jbc.M109.037614

TABLE 2.

Kinetic parameters of O. tauri ADP-Glc PPase enzymes for substrates

Enzyme S0.5 ATP n S0.5 Mg2+ n S0.5 Glc-1-P n
mm mm mm
OtaS 0.415 ± 0.052 1.58 3.65 ± 0.21 2.76 3.08 ± 0.28 0.89
OtaS/OtaL 0.373 ± 0.036 1.93 4.23 ± 0.18 3.14 0.092 ± 0.014 1.07
OtaSD148A/OtaL 0.145 ± 0.011 1.54 1.56 ± 0.07 3.40 0.047 ± 0.003 1.60
OtaS/OtaLD171A 0.491 ± 0.046 1.53 4.08 ± 0.28 2.37 0.044 ± 0.005 1.50