TABLE 3.

Kinetic parameters of O. tauri ADP-Glc PPase enzymes for activator and inhibitor

Enzymes were purified to homogeneity as described under “Experimental Procedures.” The A_0.5 and I_0.5 values for 3-PGA and P_i, respectively, are shown for O. tauri enzymes purified to homogeneity.

	A0.5 3-PGA	Fold activation	I0.5 Pia	Fold decrease
	mm		mm
OtaS	1.99 ± 0.29	6	2.34 ± 0.32	3
OtaS/OtaL	0.544 ± 0.074	33	0.64 ± 0.09	3
OtaSD148A/OtaL	0.0080 ± 0.0015	3	3.05 ± 0.87	48
OtaS/OtaLD171A	0.059 ± 0.010	8	0.137 ± 0.017	3

^a Concentration of 3-PGA was at their respective A_0.5 values.