FIGURE 2.
High throughput G17 binding data for microarrays of overlapping CDR-derived bicyclic peptidomimetics. A, amino acid sequences for the CDRs of five anti-G17 mAbs/scFvs + G17 affinity data as determined by SPR or enzyme-linked immunosorbent assay (only PAR10C3); gray areas indicate CDR regions covered in microarray synthesis. B, experimental design of microarrays with overlapping bicyclic peptidomimetics (surface-immobilized) derived from the H2, H3, and L3 CDRs of the five anti-G17 antibodies. C–G, A450 nm values for G17 binding to bicyclic peptidomimetics derived from mAb 243BA5 (C), mAb 23CA8 (D), mAb 189DB3 (E), scFv PAR10C3 (F), and scFv PAR10D10 (G). Binding studies were performed with 10 μg/ml of biotinylated G17 (pyroEGPWLEEEEEAYGWMDFK(biotin), followed by conjugation using peroxidase-labeled streptavidin at 1:1000 dilution. nr, number.