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. 2009 Oct 6;284(49):34126–34134. doi: 10.1074/jbc.M109.041459

FIGURE 4.

FIGURE 4.

Analysis of G17 binding strength of CDR-derived bicyclic peptidomimetics. A–C, correlation of G17 binding levels (A450 nm values) to overall charge of bicyclic peptidomimetics derived from mAb 189DB3 (A), scFv PAR10C3 (B), and scFv PAR10D10 (C). D, complete Asp scan and Lys-10 replacement analysis of bicyclic peptidomimetic 1e (CTKSGGSCTVASIKCT-T3) (derived from mAb 189DB3), showing the crucial role of charge in G17 recognition. E, comparison of G17 binding levels (A450 nm) in PEPSCAN microarrays and SPR (%Rmax, being 100% Rmax when Req = Rmax,calc) in relation to overall charge for some of the best and worst G17 binders derived from mAb 189DB3, scFv PAR10C3, and scFv PAR10D10.