FIGURE 2.

Characterization of human NF-YC promoters. A, University of California Santa Cruz genomic view of human NF-YC promoters region (30-kb window, left plot). A 4-kb zoom of the P2 region is also shown (right plot). B, sequence alignments of human and mouse P1 and P2 regions, 500 bp upstream of the transcription start site (arrow). P2 CCAAT boxes are shaded gray. C, basal activity of P1 and P2 regions in HCT116 cells. 500 ng of each reporter construct were cotransfected with 100 ng of pCMV β-galactosidase, and normalized luciferase (Lucif) units were calculated 36 h after transfection. The structure of NF-YC P1 and P2 reporter constructs is also depicted (bottom panel).