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. 2009 Aug 18;284(49):34189–34200. doi: 10.1074/jbc.M109.008417

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — NF-YC P2 and 37-kDa NF-YC are induced by DNA damage. A, HCT116 and HCT116/E6 were treated with doxorubicin (Doxo) (1 μm) for the indicated time points, and semi-quantitative RT-PCR analyses were performed for the indicated genes, after normalizing samples for β-actin content (upper panel). RT-PCRs were performed in the linear range of amplification; number of cycles for each product is indicated in the middle. B, Western blot analysis was performed with anti-NF-YC antibody on nuclear extracts from HCT116 and HCT116/E6 treated with 1 μm doxorubicin for the indicated time points. NF-YB was used as an internal control for nuclear extracts loading (lower panel). C, ChIP analysis and semi-quantitative PCRs were performed for NF-YC P2 promoter in HCT116 cells, before and 4 h after 1 μm doxorubicin, with anti-p53 Ab7 (polyclonal) and anti-p53 DO1 (monoclonal) antibodies. The anti-FLAG antibody was used as a negative control. ChIP PCRs were performed in the linear range for each amplification product.