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. 2009 Aug 18;284(49):34189–34200. doi: 10.1074/jbc.M109.008417

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Functional inactivation of the 37-kDa isoform. The upper panel indicates the position in exon 8 and exon 9 of the shRNA used to functionally inactivate the 37-kDa isoform. HCT116 were infected with lentiviruses producing control and shRNA37, and treated with doxorubicin (1 μm) for 8 h. Semi-quantitative RT-PCR analysis was performed for the indicated genes, after normalizing samples for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (lower panel) and β-actin (data not shown). RT-PCRs were performed in the linear range of amplification.