FIGURE 10.

Characterizing the interaction between the CT and CL domains of Cx43 and Cx40 from the CT point of view. These experiments allowed for detection of the regions of Cx43CT or Cx40CT that were structurally modified when in the presence of a binding domain from the molecular partners Cx43CL and Cx40CL. The Cx43CT was titrated with the Cx40CL (panel A) or Cx43CL (panel B) domains to a 1:35 molar ratio. The Cx40CT was titrated with the Cx40CL domain (panel C) to a 1:35 molar ratio. ¹⁵N-HSQC spectra for Cx43CT or Cx40CT alone (black) have been overlaid with spectra obtained in the presence of the Cx40CL or Cx43CL domains (red). Below each panel is a close-up of resonance peaks from the box in each panel.