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. 2009 Oct 6;284(49):34283–34295. doi: 10.1074/jbc.M109.034462

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — NSD2 carried out dimethylation of H3K36 in vivo. siRNA silencing experiment was carried out in HeLa cells using two different siRNA oligos against NSD2 and a control (Ctr) siRNA oligo. A, nuclear extracts of the siRNA-treated cells were prepared and analyzed by Western blot using antibodies against NSD1, NSD2, and β-tubulin. Histones were acid-extracted from the treated cells and analyzed by Western blot using the antibodies specified. B, histones isolated from cells treated with NSD2- or control (Ctr)-siRNA were loaded in two amounts, and the methylation levels on H3K36 and H4K20 were analyzed by Western blot using specific antibodies.