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. 2009 Sep 18;284(49):34366–34375. doi: 10.1074/jbc.M109.053017

FIGURE 3.

FIGURE 3.

MecA-(121–218) requires an N-terminal flexible sequence for ClpCP degradation. A, systematic deletion analysis of MecA. MecA-(121–218) appears to require extra 9 amino acids at its N terminus in order for itself to be degraded by ClpCP. B, the degradation of the chimeric protein between the BIR1 domain and MecA-(121–218) requires an N-terminal flexible sequence. Deletion of 8 amino acids from the N terminus of the BIR1 domain (residues 31–145) led to abrogation of degradation (lanes 5–7). By contrast, removal of internal sequences in BIR1 had no impact on degradation (lanes 1–3). C, the degradation of GST requires an N-terminal flexible sequence. GST was degraded only when it was fused C-terminal to MecA-(96–218) (left panel). GST fused N-terminal to MecA-(96–218) resisted degradation, presumably due to the lack of flexible sequences at its N terminus.