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Dynamic nature of the MecA-ClpCP system. A, the constant unfolding and degradation of MecA by the ClpCP protease following MecA-mediated assembly of the ClpCP protease. MecA-(96–218) was fused with GFP to create a substrate protein. Fluorescence intensity was monitored as a convenient indicator of unfolding and degradation. B, the degradation of MecA results in the disassembly of the functional ClpCP protease. mAU, milliabsorbance units.
