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. 2009 Oct 8;284(49):34433–34443. doi: 10.1074/jbc.M109.036202

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Down-regulation of seladin-1 decreases co-localization of BACE1-Myc with the lysosomal marker LAMP2a. Confocal microscopy of subcellular localization of BACE1-Myc was assessed using anti-Myc (green) and LAMP2a (red) antibody staining in untreated (UNT) and STS-treated SH-SY5Y-BACE1-Myc cells transfected with seladin-1 (siSel) or control (siCtrl) siRNA. In siCtrl-transfected cells (with or without STS), there is some intracellular co-localization (yellow) of the two proteins, most likely in lysosomes. After seladin-1 knockdown, no apparent co-localization of BACE1-Myc with LAMP2a was detected, and more BACE1-Myc was localized near or at the plasma membrane (arrow) during apoptosis. Images show single optical z-sections.