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Mammalian peroxidase Gpx4, but not Prx6, oxidizes fused roGFP2. roGFP2 fusion proteins of glutathione peroxidase 4 (roGFP2-Gpx4) and peroxiredoxin-6 (Prx6-roGFP2) were transiently expressed in HeLa cells. Oxidation in response to 50 μm H2O2 was monitored by live cell microscopy.
