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. 2009 Sep 17;284(46):31572–31578. doi: 10.1074/jbc.M109.057927

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — CD9P-1 and EWI-2 are CD81 major partners in mouse hepatocytic cells. After cell surface biotinylation, Hepa1-6 cells were lysed with digitonin, and immunoprecipitations (IP) were performed as indicated on the top of each lane. The antibodies used for immunoprecipitations were MFR5 (Int. α5), a polyclonal antibody to EWI-2, 8G1 (CD9P-1), MT81 (CD81), and 4.1. F12 (CD9). The proteins in the immunoprecipitates were revealed using IRdye800-conjugated streptavidin. Bands corresponding to CD9P-1, EWI-2, CD81, and CD9 are indicated on the left. In the lower panel, the presence of CD81 in the different IPs was analyzed by Western blot using Alexa 680-conjugated CD81 antibody (MT81). CD81 is present in both CD9P-1 and EWI-2 IP but not in the integrin α5 (Int. α5) IP.