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. 2009 Sep 17;284(46):31572–31578. doi: 10.1074/jbc.M109.057927

FIGURE 6.

FIGURE 6.

A CD9P-1 mutant defective for the association with CD81 has no effect on sporozoite infection of hepatocytic cells. A, Hepa1-6 cells were transiently transfected with plasmids coding for CD9P-1, CD9P-1/HLA, CD9P-1/CytHLA, EWI-2, or empty vector (mock), 24 h before infection with P. yoelii sporozoites. The number of EEF-infected cells (mean ± S.D.) was determined as described under “Experimental Procedures.” **, p < 0.01 as compared with control cells. B, analysis of interaction between human CD9P-1 and endogenous mCD81. Hepa1-6 cells were transiently transfected with CD9P-1, CD9P-1/HLA, or empty vector (mock). After 48 h, the cells were lysed in 1% Brij97 before immunoprecipitation with mAbs against mCD81 (MT81), hCD9P-1 (1F11) or mouse integrin α5 (Int.α5) as a control, as indicated at the top of each lane. Immunoprecipitates were analyzed by Western blotting using biotin-labeled mAbs (hCD9P-1, mCD81) and Alexa 680-labeled streptavidin.