FIGURE 4.

Deconvolution of spectra. A, absorption spectrum of wild type GadB at pH 6.84 resolved into its component absorption bands. B, absorption spectrum of GadBH465A at pH 8.5 resolved into its component absorption bands. The deconvolution of the spectrum of GadBΔHT at pH 8.5 gave results identical to those for GadBH465A. The protein concentration was 7.7 μm. The spectra were recorded in 50 mm potassium phosphate buffers at the indicated pH. The solid lines represent the experimental spectra, the dashed lines are the component bands, and the dotted curve is the theoretical curve obtained by nonlinear least square fit of the experimental points to the sum of a variable number of log normal curves, each having independent parameters.