Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of pH on the specific activity of wild type GadB and His465 mutants. Glutamate decarboxylase activity (units/mg) as a function of pH was measured using a pH-stat device (see “Experimental Procedures”) in a buffer-free system or in the presence of phosphate buffer (inset). Filled squares, wild type GadB; filled triangles, GadBH465A; empty circles, GadBΔHT. The solid lines through the experimental points represent the theoretical curves obtained using the Hill equation.
